NMR spectroscopy of lipidic cubic phases

Sunnia Rajput; Shenggen Yao; David W Keizer; Marc-Antoine Sani; Frances Separovic

doi:10.1007/s12551-021-00900-y

NMR spectroscopy of lipidic cubic phases

Biophys Rev. 2021 Nov 11;14(1):67-74. doi: 10.1007/s12551-021-00900-y. eCollection 2022 Feb.

Authors

Sunnia Rajput¹, Shenggen Yao¹, David W Keizer¹, Marc-Antoine Sani^{1

2}, Frances Separovic^{1

2}

Affiliations

¹ Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC 3052 Australia.
² School of Chemistry, University of Melbourne, Melbourne, VIC 3010 Australia.

Abstract

Lipidic cubic phase (LCP) structures have been used for stabilisation and crystallisation of membrane proteins and show promising properties as drug carriers. In this mini-review, we present how NMR spectroscopy has played a major role in understanding the physico-chemical properties of LCPs and how recent advances in pulsed field gradient NMR techniques open new perspectives in characterising encapsulated molecules.

Keywords: Cubic phase; Diffusion; Encapsulation; Membrane protein; NMR; Pulsed field gradient.

Publication types

Review