Relationship of citrulline and tissue transglutaminase antibody with duodenal histopathology among children with celiac disease

Parisa Rahmani; Ghobad Heidari; Fatemeh Farahmand; Alireza Moradzadeh

doi:10.1016/j.amsu.2022.103489

Relationship of citrulline and tissue transglutaminase antibody with duodenal histopathology among children with celiac disease

Ann Med Surg (Lond). 2022 Mar 15:76:103489. doi: 10.1016/j.amsu.2022.103489. eCollection 2022 Apr.

Authors

Parisa Rahmani¹, Ghobad Heidari², Fatemeh Farahmand³, Alireza Moradzadeh^{2

3}

Affiliations

¹ Pediatric Gastroenterology and Hepatology Research Center, Children's Medical Center, Pediatric Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran.
² Department of Pediatric, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
³ Department of Pediatric Gastroenterology, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Objectives: Non-invasive biomarkers, for the diagnosis of celiac disease, can reduce the need for biopsy, particularly in pediatric patients. The aim of this study was to investigate the levels tissue transglutaminase antibody (tTG) and plasma citrulline and its correlation with intestinal biopsy.

Methods: In this cross-sectional descriptive study, Pediatric patients with celiac disease referred to (XXX)were included. The patients underwent tTG antibody test along with plasma citrulline measurements using HPLC ((high performance liquid chromatography). Biopsy was performed in all the patients and clinical and demographic findings were recorded in a patient form. The data were statistically analyzed using SPSSv22.

Results: Of 118 patients with celiac disease, the mean level of citrulline in patients was 17.48 ± 6.92 and the mean tTG titer was 183.17 ± 41.25. The two variables were inversely correlated with each other, p < 0.01. With an increase in Marsh levels, a significant reduction in citrulline levels and an increase in plasma tTG levels were seen, p < 0.01, respectively. The mean citrulline and tTG titer was not associated with gender and the age of the patients.

Conclusion: Our findings indicate that citrulline and tTG antibody titer are significant biomarkers for the diagnosis of celiac disease and the severity of intestinal atrophy among pediatric patients.

Keywords: Antibody; Celiac disease; Citrulline; GFD, gluten-free diet; HLA, Human leukocyte antigen; HPLC, high-performance liquid chromatography; Pediatric; Plasma; Tissue transglutaminase antibody (tTG); tTG, tissue transglutaminase.