Silver-loaded carboxymethyl cellulose nonwoven sheet with controlled counterions for infected wound healing

Seiichi Ohta; Kento Mitsuhashi; Arvind K Singh Chandel; Pan Qi; Noriko Nakamura; Akiko Nakamichi; Hiromi Yoshida; Gento Yamaguchi; Yuichi Hara; Ryo Sasaki; Masaya Fuke; Madoka Takai; Taichi Ito

doi:10.1016/j.carbpol.2022.119289

Silver-loaded carboxymethyl cellulose nonwoven sheet with controlled counterions for infected wound healing

Carbohydr Polym. 2022 Jun 15:286:119289. doi: 10.1016/j.carbpol.2022.119289. Epub 2022 Feb 26.

Authors

Affiliations

¹ Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Institute of Engineering Innovation, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan; Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
² Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
³ Institute of Engineering Innovation, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan.
⁴ Biomaterial Business Development Department, Asahi Kasei Corporation, Hibiya Mitsui Tower 1-1-2 Yurakucho, Chiyoda-ku, Tokyo 100-0006, Japan.
⁵ Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
⁶ Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: taichi@m.u-tokyo.ac.jp.

PMID: 35337531
DOI: 10.1016/j.carbpol.2022.119289

Abstract

Carboxymethyl cellulose (CMC) is a promising material for moist wound healing, and silver loading onto CMC has been examined for anti-bacterial activity. In this study, we developed silver-loaded CMC nonwoven sheets with different counterions, namely sodium CMC (CMC-Na/Ag) and partially protonated CMC (CMC-H/Ag), to examine their anti-bacterial and wound-healing properties. Owing to the presence of counter protons, CMC-H/Ag showed slower water adsorption, dissolution, and Ag release than CMC-Na/Ag. In addition, CMC-H/Ag and CMC-Na/Ag exhibited differences in anti-bacterial activities in shake-flask and inhibition zone tests in vitro. An in vivo experiment using a pressure ulcer mouse model with Pseudomonas aeruginosa infection showed that CMC-Na/Ag significantly accelerated wound healing compared to CMC-H/Ag and a commercially available Ag-loaded CMC nonwoven sheet, Aquacel Ag. These results suggest the importance of controlling CMC counterions and the therapeutic potential of the developed product as a wound dressing.

Keywords: Anti-bacterial; Carboxymethyl cellulose; Counterions; Silver release; Wound dressings; Wound healing.

MeSH terms

Animals
Bandages
Carboxymethylcellulose Sodium / pharmacology
Mice
Silver* / pharmacology
Silver* / therapeutic use
Wound Healing
Wound Infection* / drug therapy

Substances

Silver
Carboxymethylcellulose Sodium