Maritimibacter dapengensis sp. nov., a poly- β-hydroxyalkanoates-producing bacterium isolated from sediment of the Dapeng peninsula (Guangdong, China)

Abstract

A Gram-negative, obligate aerobic, non-motile, rod-shaped bacterium lacking a flagellum, was isolated from sediment sampled at the Dapeng peninsula of Shenzhen, Guangdong, China, and designated strain DP4N28-5^T. Growth of strain DP4N28-5^T occurred at 15-45 °C (optimum at 30 °C), pH 6.0-8.0 (optimum at 6.0) and in 2.0-10.0 % NaCl (w/v; optimum at 2.0-3.0 %). The 16S rRNA gene sequence analysis revealed that strain DP4N28-5^T was most closely related to Maritimibacter alkaliphilus DSM 100037^T (97.6 %; sequence similarity). The strain synthesized a trace amount of poly-β-hydroxybutyrate. The dominant cellular fatty acids were summed feature 8 (C_{18 : 1} ω6c and/or C_{18 : 1} ω7c), C_{16 : 0}, 11-mehtyl C_{18 : 1} ω7c and C_{16 : 0} 2-OH. The major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, one unidentified aminophospholipid, three unidentified aminolipids and four unidentified lipids. The respiratory quinone was Q-10. The DNA G+C content was 62.0 mol% and the estimated genome size was 3.9 Mbp. The calculated average nucleotide value between strain DP4N28-5^T and M. alkaliphilus DSM 100037^T was 78.1 %. The phenotypic, genotypic and chemotaxonomic differences between strain DP4N28-5^T and its phylogenetic relatives indicate that DP4N28-5^T represents a novel species in the genus Maritimibacter, for which the name Maritimibacter dapengensis sp. nov. is proposed. The type strain is DP4N28-5^T (=MCCC 1K05640^T=KCTC 82805^T). The description of genus Maritimibacter was emended to include this new species.

Keywords: ANI; Maritimibacter; draft genome sequencing; poly-β-hydroxyalkanoates; sediment.