Setting appropriate reference conditions (RCs) is critical for classifying the Ecological Quality Status (EcoQS) which is extremely challenging, considering the present-day estuaries. The EcoQS of three tropical estuaries was assessed by applying five different RCs to identify the best applicable method for the area. The AZTI's indices (AMBI and M-AMBI) categorised areas with sharper pollution gradient with ease, while classification of moderate-low polluted areas was ambiguous. Indices responded to chemical stressors more clearly in the impacted estuary compared to the less polluted estuaries. Ecological status assigned by the default RC (lowest AMBI and highest diversity and richness values) were more accurate than those obtained on application of other four types of RCs, owing to various estuarine constraints that are discussed herewith. Thus, prior to application, caution should be exercised while setting area-specific RCs. The inclusion of combination of AZTI's indices with professional judgment for successful appraisal of ecosystem is recommended.
Keywords: Benthic indices; Ecological quality status; Macroinvertebrates; Reference conditions; Salinity zones; Tropical estuaries.
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