Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control

Tao Zhang; Fei Wang; Hui-Shan Guo; Yun Jin

doi:10.1007/978-1-0716-1875-2_16

Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control

Methods Mol Biol. 2022:2408:243-252. doi: 10.1007/978-1-0716-1875-2_16.

Authors

Tao Zhang¹, Fei Wang¹, Hui-Shan Guo^{1

2}, Yun Jin³

Affiliations

¹ State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
² CAS Center for Excellence in Biotic Interactions, University of the Chinese Academy of Sciences, Beijing, China.
³ State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. jiny@im.ac.cn.

PMID: 35325427
DOI: 10.1007/978-1-0716-1875-2_16

Abstract

Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in cotton plants to confer Verticillium wilt disease resistance. Here, we provide a detailed protocol of cotton infection with V. dahliae, fungal hyphae recovery from infected cotton stems, and transmitted small RNA detection developed from our previous studies for trans-kingdom RNAi assays.

Keywords: Cotton infection; Fungal hyphae recovery; Trans-kingdom small RNA; Verticillium dahliae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance / genetics
Mycoses*
Plant Diseases / genetics
Plant Diseases / microbiology
RNA Interference
Verticillium* / genetics