High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

Ramegowda Yamunarani; Venkategowda Ramegowda; Muthappa Senthil-Kumar; Kirankumar S Mysore

doi:10.1007/978-1-0716-1875-2_12

High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

Methods Mol Biol. 2022:2408:181-189. doi: 10.1007/978-1-0716-1875-2_12.

Authors

Ramegowda Yamunarani¹, Venkategowda Ramegowda², Muthappa Senthil-Kumar³, Kirankumar S Mysore⁴

Affiliations

¹ Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, India.
² Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore, India. ramshv@gmail.com.
³ National Institute of Plant Genome Research, New Delhi, India.
⁴ Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK, USA.

PMID: 35325423
DOI: 10.1007/978-1-0716-1875-2_12

Abstract

The high throughputness and affordability of "omics" technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo functional characterization of these genes under multiple stresses is challenging but essential to develop resilient crops for the changing climate. Here we describe a high-throughput Virus-Induced Gene Silencing-based methodology for functional analysis of genes under multiple abiotic stresses using leaf disks. Leaves with maximal silencing, which is localized to only a few leaves and to a short period, can be effectively used for multiple stress imposition and stress affect quantification.

Keywords: Leaf disks; Multiple abiotic stresses; Virus-Induced Gene Silencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Plant*
Phenotype
Plant Leaves / genetics
Plants / genetics
Stress, Physiological* / genetics