We report detecting infectious Toscana virus in the seminal fluid of a 25-year-old man from Italy returning from Elba Island. The presence of infectious virus in human semen adds Toscana virus to the long list of viruses detected in this genital fluid and indicates a potential for sexual transmission.
Keywords: Italy; Toscana virus; genital tropism; meningitis/encephalitis; seminal fluid; vector-borne infections; viral isolation; viral persistence; viruses.