Long non-coding RNA PAXIP-AS1 promotes viability, invasion, and migration of HTR-8/SVneo cells through miR-210-3p/BDNF axis

Xuejuan Chen; Xiang Kong; Gang Niu; Fengjin Qin; Yan Duan; Fengjiao Ren

doi:10.1080/10641955.2022.2056194

Long non-coding RNA PAXIP-AS1 promotes viability, invasion, and migration of HTR-8/SVneo cells through miR-210-3p/BDNF axis

Hypertens Pregnancy. 2022 May;41(2):107-115. doi: 10.1080/10641955.2022.2056194. Epub 2022 Mar 22.

Authors

Xuejuan Chen¹, Xiang Kong¹, Gang Niu², Fengjin Qin², Yan Duan¹, Fengjiao Ren¹

Affiliations

¹ Department of Gynecology and Obstetrics, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, China.
² Department of Reproductive Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, China.

PMID: 35317685
DOI: 10.1080/10641955.2022.2056194

Abstract

Objective: The aim of this research was to explore the role and potential mechanism of long non-coding RNA PAXIP-AS1 in preeclampsia.

Methods: To investigate the effects of PAXIP-AS1 on cell viability, migration, and invasion. The miR-210-3p-targeted relationship with lncRNA PAXIP-AS1 or BDNF was verified.

Results: PAXIP-AS1 was inversely correlated with miR-210-3p and BDNF was targeted by miR-210-3p. BDNF was positively correlated with PAXIP-AS1 in the serum of preeclampsia patients. The promotion effects of PAXIP-AS1 on cell viability, migration, and invasion were reversed by miR-210-3p up-regulation or BDNF knockdown in trophoblast cells.

Conclusion: PAXIP-AS1 promoted the viability, migration, and invasion of trophoblast cells by regulating the miR-210-3p/BDNF axis.

Keywords: PAXIP-AS1; Preeclampsia; brain-derived neurotrophic factor; miR-210-3p.

MeSH terms

Brain-Derived Neurotrophic Factor
Cell Movement / genetics
Cell Proliferation / genetics
Female
Humans
MicroRNAs* / genetics
Pre-Eclampsia* / genetics
Pregnancy
RNA, Long Noncoding* / genetics

Substances

Brain-Derived Neurotrophic Factor
MIRN210 microRNA, human
MicroRNAs
RNA, Long Noncoding