Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

Yuhan Pan; Juan Zhu; Yi Hong; Mengna Zhang; Chao Lv; Baojian Guo; Huiquan Shen; Xiao Xu; Rugen Xu

doi:10.7717/peerj.13128

Screening of stable resistant accessions and identification of resistance loci to Barley yellow mosaic virus disease

PeerJ. 2022 Mar 17:10:e13128. doi: 10.7717/peerj.13128. eCollection 2022.

Authors

Yuhan Pan¹, Juan Zhu¹, Yi Hong¹, Mengna Zhang¹, Chao Lv¹, Baojian Guo¹, Huiquan Shen², Xiao Xu², Rugen Xu¹

Affiliations

¹ Yangzhou University, Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Institutes of Agricultural Science, Yangzhou, Jiangsu, China.
² Jiangsu Institute for Seaside Agricultural Sciences and Yancheng Academy of Agricultural Science, Yancheng, Jiangsu, China.

Abstract

Background: The disease caused by Barley yellow mosaic virus (BaYMV) infection is a serious threat to autumn-sown barley (Hordeum vulgare L.) production in Europe, East Asia and Iran. Due to the rapid diversification of BaYMV strains, it is urgent to discover novel germplasm and genes to assist breeding new varieties with resistance to different BaYMV strains, thus minimizing the effect of BaYMV disease on barley cropping.

Methods: A natural population consisting of 181 barley accessions with different levels of resistance to BaYMV disease was selected for field resistance identification in two separate locations (Yangzhou and Yancheng, Jiangsu Province, China). Additive main effects and multiplicative interaction (AMMI) analysis was used to identify accessions with stable resistance. Genome-wide association study (GWAS) of BaYMV disease resistance was broadly performed by combining both single nucleotide polymorphisms (SNPs) and specific molecular markers associated with the reported BaYMV disease resistance genes. Furthermore, the viral protein genome linked (VPg) sequences of the virus were amplified and analyzed to assess the differences between the BaYMV strains sourced from the different experimental sites.

Results: Seven barley accessions with lower standardized Area Under the Disease Progress Steps (sAUDPS) index in every environment were identified and shown to have stable resistance to BaYMV disease in each assessed location. Apart from the reported BaYMV disease resistance genes rym4 and rym5, one novel resistance locus explaining 24.21% of the phenotypic variation was identified at the Yangzhou testing site, while two other novel resistance loci that contributed 19.23% and 19.79% of the phenotypic variation were identified at the Yancheng testing site, respectively. Further analysis regarding the difference in the VPg sequence of the predominant strain of BaYMV collected from these two testing sites may explain the difference of resistance loci differentially identified under geographically distinct regions. Our research provides novel genetic resources and resistance loci for breeding barley varieties for BaMYV disease resistance.

Keywords: Additive main effects and multiplicative interaction (AMMI); Barley (Hordeum vulgare L.); Barley yellow mosaic disease (BYMD); Genome-wide association study (GWAS); Stable resistant accessions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance* / genetics
Genome-Wide Association Study
Plant Breeding
Potyviridae* / genetics

Supplementary concepts

Barley yellow mosaic virus

Grants and funding

The authors received funding from the National key R&D Program of China (2018YFD1000700), National key R&D Program of China (2018YFD1000703), National Modern Agriculture Industry Technology System, China (CARS-05), Jiangsu University Advantage Discipline Construction Project and Jiangsu Agricultural Science and Technology Innovation Fund (CX(19)3062). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.