Confocal laser endomicroscopy and confocal microscopy for head and neck cancer imaging: Recent updates and future perspectives

Adrien Villard; Ingrid Breuskin; Odile Casiraghi; Safaa Asmandar; Corinne Laplace-Builhe; Muriel Abbaci; Antoine Moya Plana

doi:10.1016/j.oraloncology.2022.105826

Confocal laser endomicroscopy and confocal microscopy for head and neck cancer imaging: Recent updates and future perspectives

Oral Oncol. 2022 Apr:127:105826. doi: 10.1016/j.oraloncology.2022.105826. Epub 2022 Mar 19.

Authors

Adrien Villard¹, Ingrid Breuskin², Odile Casiraghi³, Safaa Asmandar⁴, Corinne Laplace-Builhe⁵, Muriel Abbaci⁶, Antoine Moya Plana⁷

Affiliations

¹ Head and Neck Oncology Department, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France. Electronic address: adrien.villard@gustaveroussy.fr.
² Head and Neck Oncology Department, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France. Electronic address: ingrid.breuskin@gustaveroussy.fr.
³ Pathology Department, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France. Electronic address: odile.casiraghi@gustaveroussy.fr.
⁴ Pathology Department, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France.
⁵ Imaging and Cytometry Platform, IRCIV, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France; Paris Saclay University, UMR CNRS 8081-IR4M, Orsay F-91401, France. Electronic address: corinne.laplace@gustaveroussy.fr.
⁶ Imaging and Cytometry Platform, IRCIV, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France; Paris Saclay University, UMR CNRS 8081-IR4M, Orsay F-91401, France. Electronic address: muriel.abbaci@gustaveroussy.fr.
⁷ Head and Neck Oncology Department, Gustave Roussy Cancer Campus, 114 rue Edouard Vaillant, Villejuif 94805, France. Electronic address: antoine.moya-plana@gustaveroussy.fr.

PMID: 35316771
DOI: 10.1016/j.oraloncology.2022.105826

Abstract

Confocal laser endomicroscopy (CLE) is a promising technology that provides real-time histological visualization of living tissue, described as "optical biopsy". It has been used successfully in dermatology, gastroenterology and ophthalmology for clinical diagnosis and tumor margin assessment. In addition to in vivo tissue assessment, confocal microscopy can also be used ex vivo on freshly excised or fixed tissue for intraoperative tumor margin assessment. While CLE and ex vivo confocal microscopy (EVCM) have shown promising results for the identification of head and neck cancers at cell level, several methodological issues need to be addressed before they can be widely used in routine clinical use. The present review will focus on recent advances in CLE and EVCM in the diagnosis and management of head and neck cancer and discuss remaining challenges for their clinical application.

Trial registration: ClinicalTrials.gov NCT01626638.

Keywords: Confocal laser endomicroscopy; Ex vivo confocal microscopy; Fluorescence; Head and neck cancer; Optical biopsy; Reflectance; Squamous Cell Carcinoma.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Endoscopy
Head and Neck Neoplasms* / diagnostic imaging
Humans
Lasers
Margins of Excision
Microscopy, Confocal / methods

Associated data

ClinicalTrials.gov/NCT01626638