Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Fatima Mekhalif; Rita Zgheib; Jean Akiana; Melhem Bilen; Geor Mongo Ndombe; Florence Fenollar; Pierre-Edouard Fournier; Didier Raoult; Stéphane Alibar; Oleg Mediannikov; Cheikh Ibrahima Lo

doi:10.1016/j.heliyon.2022.e09102

Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Heliyon. 2022 Mar 14;8(3):e09102. doi: 10.1016/j.heliyon.2022.e09102. eCollection 2022 Mar.

Authors

Fatima Mekhalif^{1

2}, Rita Zgheib^{2

3}, Jean Akiana⁴, Melhem Bilen^{1

3}, Geor Mongo Ndombe⁴, Florence Fenollar^{2

5}, Pierre-Edouard Fournier^{2

5}, Didier Raoult^{1

2}, Stéphane Alibar², Oleg Mediannikov^{1

2}, Cheikh Ibrahima Lo^{2

5}

Affiliations

¹ Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
² IHU-Méditerranée Infection, Marseille, France.
³ University of Balamand, Faculty of Medicine and Medical Sciences, Amioun, Lebanon.
⁴ Laboratoire National de Santé Publique, Brazzaville, Congo.
⁵ Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.

Abstract

The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308^T from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308^T is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460^T (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308^T with its closest related species was 71.1%, which was far below the recommended threshold (>95-96%). The genome of the strain Marseille-P4308^T has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308^T (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed.

Keywords: Culturomics; Gut microbiote; Indigenous congolese; Peptostreptococcus faecalis; Taxonogenomics.