Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice

Huijuan Zhang; Dewei Zheng; Fengming Song; Ming Jiang

doi:10.3389/fpls.2022.840360

Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice

Front Plant Sci. 2022 Mar 2:13:840360. doi: 10.3389/fpls.2022.840360. eCollection 2022.

Authors

Huijuan Zhang¹, Dewei Zheng¹, Fengming Song², Ming Jiang¹

Affiliations

¹ College of Life Science, Taizhou University, Taizhou, China.
² National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, China.

Abstract

E3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice were found. Their functions were analyzed in rice. These 11 E3 ubiquitin ligase genes showed different patterns of expression under different treatments. The BMV:OsPUB39-infiltrated seedlings showed decreased resistance to Magnaporthe grisea (M. grisea) when compared with BMV:00-infiltrated seedlings, whereas the BMV:OsPUB34- and BMV:OsPUB33-infiltrated seedlings showed increased resistance. The involvement of these genes in the resistance against M. grisea may be attributed to the regulation of the accumulation of reactive oxygen species (ROS) and expression levels of defense-related genes. Seedlings infiltrated by BMV:OsATL69 showed decreased tolerance to drought stress, whereas BMV:OsPUB33-infiltraed seedlings showed increased tolerance, possibly through the regulation of proline content, sugar content, and expression of drought-responsive genes. BMV:OsATL32-infiltrated seedlings showed decreased tolerance to cold stress by regulating malondialdehyde (MDA) content and the expression of cold-responsive genes.

Keywords: E3 ubiquitin ligases; ROS; abiotic stress; biotic stress; expression patterns; rice.