Facile Fabrication of 3D-Printed Porous Ti6Al4V Scaffolds with a Sr-CaP Coating for Bone Regeneration

Shenghui Su; Weidong Chen; Minghui Zheng; Guozan Lu; Wei Tang; Haihong Huang; Dongbin Qu

doi:10.1021/acsomega.1c05908

Facile Fabrication of 3D-Printed Porous Ti6Al4V Scaffolds with a Sr-CaP Coating for Bone Regeneration

ACS Omega. 2022 Mar 1;7(10):8391-8402. doi: 10.1021/acsomega.1c05908. eCollection 2022 Mar 15.

Authors

Shenghui Su¹, Weidong Chen¹, Minghui Zheng^{1

2}, Guozan Lu³, Wei Tang⁴, Haihong Huang¹, Dongbin Qu^{1

2}

Affiliations

¹ Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 510515 Guangzhou, China.
² Department of Orthopaedic Surgery, Zengcheng Branch of Nanfang Hospital, Southern Medical University, 511338 Guangzhou, China.
³ Guangzhou Huatai 3D Material Manufacture Ltd., Co., 511300 Guangzhou, China.
⁴ Department of Anatomy, College of Basic Medicine, Dalian Medical University, Dalian 116044, China.

Abstract

To improve osseointegration caused by the stress-shielding effect and the inert nature of titanium-based alloys, in this work, we successfully constructed a strontium calcium phosphate (Sr-CaP) coating on three-dimensional (3D)-printed Ti6Al4V scaffolds to address this issue. The energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) results indicated that the coatings with and without Sr doping mainly consisted of CaHPO₄. The bonding strength of Sr doping coating met the required ISO 13 779-4-2018 standard (≥15 MPa). The in vitro results suggested that the Sr-CaP-modified Ti6Al4V scaffolds were found to effectively promote mice bone-marrow stem cell (mBMSC) adhesion, spreading, and osteogenesis. The in vivo experiments also showed that the Sr-CaP-modified Ti6Al4V scaffolds could significantly improve bone regeneration and osseointegration. More importantly, Sr-doped CaP-coated Ti6Al4V scaffolds were found to accelerate bone healing in comparison to CaP-coated Ti6Al4V scaffolds. The Sr-CaP-modified Ti6Al4V scaffolds are considered a promising strategy to develop bioactive surfaces for enhancing the osseointegration between the implant and bone tissue.