Plasma and bronchoalveolar lavage fluid oxylipin levels in experimental porcine lung injury

Niklas Larsson; Stefan Lehtipalo; Sandra Gouveia-Figueira; Jonas Claesson; Jamshid Pourazar; Martin Isaksson Mettävainio; Michael Haney; Malin L Nording

doi:10.1016/j.prostaglandins.2022.106636

Plasma and bronchoalveolar lavage fluid oxylipin levels in experimental porcine lung injury

Prostaglandins Other Lipid Mediat. 2022 Jun:160:106636. doi: 10.1016/j.prostaglandins.2022.106636. Epub 2022 Mar 17.

Authors

Niklas Larsson¹, Stefan Lehtipalo², Sandra Gouveia-Figueira³, Jonas Claesson⁴, Jamshid Pourazar⁵, Martin Isaksson Mettävainio⁶, Michael Haney⁷, Malin L Nording⁸

Affiliations

¹ Anesthesiology and Intensive Care Medicine, Department of Surgical and Perioperative Sciences, Umeå University, 90185 Umeå, Sweden. Electronic address: niklas.larsson@regionvasterbotten.se.
² Anesthesiology and Intensive Care Medicine, Department of Surgical and Perioperative Sciences, Umeå University, 90185 Umeå, Sweden. Electronic address: stefan.lehtipalo@regionvasterbotten.se.
³ Department of Chemistry, Umeå University, 901 87 Umeå, Sweden. Electronic address: sandra.gouveia@regionvasterbotten.se.
⁴ Anesthesiology and Intensive Care Medicine, Department of Surgical and Perioperative Sciences, Umeå University, 90185 Umeå, Sweden. Electronic address: jonas.claesson@regionorebrolan.se.
⁵ Department of Public Health and Clinical Medicine, Division of Medicine, Umeå University, 90185 Umeå, Sweden. Electronic address: jamshid.pourazar@umu.se.
⁶ Pathology, Department of Medical Biosciences, Umeå University, 901 85 Umeå, Sweden. Electronic address: martin.isaksson.mettavainio@regionvasterbotten.se.
⁷ Anesthesiology and Intensive Care Medicine, Department of Surgical and Perioperative Sciences, Umeå University, 90185 Umeå, Sweden. Electronic address: michael.haney@umu.se.
⁸ Department of Chemistry, Umeå University, 901 87 Umeå, Sweden. Electronic address: malin.nording@umu.se.

PMID: 35307566
DOI: 10.1016/j.prostaglandins.2022.106636

Abstract

Inflammatory signaling pathways involving eicosanoids and other regulatory lipid mediators are a subject of intensive study, and a role for these in acute lung injury is not yet well understood. We hypothesized that oxylipin release from lung injury could be detected in bronchoalveolar lavage fluid and in plasma. In a porcine model of surfactant depletion, ventilation with hyperinflation was assessed. Bronchoalveolar lavage and plasma samples were analyzed for 37 different fatty acid metabolites (oxylipins). Over time, hyperinflation altered concentrations of 4 oxylipins in plasma (TXB₂, PGE₂, 15-HETE and 11-HETE), and 9 oxylipins in bronchoalveolar lavage fluid (PGF_2α, PGE₂, PGD₂, 12,13-DiHOME, 11,12-DiHETrE, 13-HODE, 9-HODE, 15-HETE, 11-HETE). Acute lung injury caused by high tidal volume ventilation in this porcine model was associated with rapid changes in some elements of the oxylipin profile, detectable in lavage fluid, and plasma. These oxylipins may be relevant in the pathogenesis of acute lung injury by hyperinflation.

Keywords: Biomarkers; Inflammation; Lung injury; Mechanical ventilation; Oxylipins; Swine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Lung Injury*
Animals
Bronchoalveolar Lavage Fluid
Dinoprostone
Eicosanoids
Oxylipins*
Swine

Substances

Eicosanoids
Oxylipins
Dinoprostone