Polyploidization affects the allelic variation of jasmonate-regulated protein Ta-JA1 belonging to the monocot chimeric jacalin (MCJ) family in wild emmer wheat

Lanxin Mei; Xiaoran Gao; Xiaoyu Yi; Mengmeng Zhao; Jinhui Wang; Zhen Li; Jiamin Li; Jian Ma; Zhien Pu; Yuanying Peng; Qiantao Jiang; Guoyue Chen; Jirui Wang; Yuming Wei; Youliang Zheng; Wei Li

doi:10.1016/j.gene.2022.146399

Polyploidization affects the allelic variation of jasmonate-regulated protein Ta-JA1 belonging to the monocot chimeric jacalin (MCJ) family in wild emmer wheat

Gene. 2022 May 30:825:146399. doi: 10.1016/j.gene.2022.146399. Epub 2022 Mar 17.

Authors

Lanxin Mei¹, Xiaoran Gao¹, Xiaoyu Yi¹, Mengmeng Zhao¹, Jinhui Wang¹, Zhen Li¹, Jiamin Li¹, Jian Ma², Zhien Pu³, Yuanying Peng², Qiantao Jiang², Guoyue Chen², Jirui Wang², Yuming Wei², Youliang Zheng², Wei Li⁴

Affiliations

¹ College of Agronomy, Sichuan Agricultural University, Chengdu, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.
² Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
³ College of Agronomy, Sichuan Agricultural University, Chengdu, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
⁴ College of Agronomy, Sichuan Agricultural University, Chengdu, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. Electronic address: liw03@163.com.

PMID: 35306115
DOI: 10.1016/j.gene.2022.146399

Abstract

The jasmonate-regulated protein Ta-JA1 belongs to the monocot chimeric jacalin (MCJ) family and plays a vital role in stress resistance in wheat. However, the impact of wheat polyploidization on Ta-JA1 remains unclear. In this study, 149 members of the MCJ family were identified among members of Triticeae using a genome-wide approach. The genes were resolved into three clades; MCJ genes in each clade were derived from different donor genes during evolution. Segmental duplication may have been the primary driver, compared with tandem duplication, of expansion in the MCJ family of wheat. Gene loss and acquisition occurred during tetraploidization, and the core expansion of the family occurred after tetraploidization. Sequencing data for 2104 accessions of T. aestivum and 99 accessions of T. dicoccoides showed that Ta-JA1-2A and Ta-JA1 were highly conserved in common wheat, and four alleles (TdJA1-Ax2, TdJA1-Ay2, TdJA1-Ax3, and TdJA1-Ay3) were detected in T. dicoccoides. Using gene-specific markers, one AsJA1-B allele was detected in 11 Ae. speltoides accessions and one TuJA1-Ax1 allele was detected in 70 T. urartu accessions. Six alleles were detected on chromosome 2A: TdJA1-Ax1 (13 accessions), TdJA1-Ay1 (57 accessions), TdJA1-Ax2 (23 accessions), TdJA1-Ay2 (42 accessions), TdJA1-Ax3 (29 accessions), and TdJA1-Ay3 (251 accessions). Only one allele (TdJA1-B) on chromosome 2B was detected in 415 T. dicoccoides accessions. A geographical distribution analysis revealed that Israel hosted higher allelic variation than other regions. Quantitative reverse transcription PCR analysis indicated that divergence in expression has occurred among Ta-JA1 alleles and, notably, TdJA1-Ax1 and TdJA1-Ay1 showed significantly higher expression levels than the other four allelic types in T. dicoccoides. The present results contribute to an improved understanding of the effects of polyploidization on the MCJ gene family and the functions of Ta-JA1, and may be useful to enrich common wheat germplasm resources.

Keywords: MCJ gene; Polyploidization; Ta-JA1; Triticum dicoccoides; Wheat evolution.

MeSH terms

Alleles
Cyclopentanes
Oxylipins* / metabolism
Plant Lectins
Triticum* / genetics
Triticum* / metabolism

Substances

Cyclopentanes
Oxylipins
Plant Lectins
jacalin
jasmonic acid