Structural characterization of a polysaccharide from Trametes sanguinea Lloyd with immune-enhancing activity via activation of TLR4

Mengting Zhang; Mengxia Yan; Jiaqian Yang; Fenfen Li; Yiran Wang; Kaiyuan Feng; Sanying Wang; Nengming Lin; Yiqi Wang; Bo Yang

doi:10.1016/j.ijbiomac.2022.03.072

Structural characterization of a polysaccharide from Trametes sanguinea Lloyd with immune-enhancing activity via activation of TLR4

Int J Biol Macromol. 2022 May 1:206:1026-1038. doi: 10.1016/j.ijbiomac.2022.03.072. Epub 2022 Mar 17.

Authors

Mengting Zhang¹, Mengxia Yan¹, Jiaqian Yang¹, Fenfen Li¹, Yiran Wang¹, Kaiyuan Feng¹, Sanying Wang², Nengming Lin³, Yiqi Wang⁴, Bo Yang⁵

Affiliations

¹ School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 260 Baichuan Street, Hangzhou 311402, PR China.
² Zhejiang Provincial Key Lab of Geriatrics, Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310030, PR China.
³ Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, PR China. Electronic address: lnm1013@zju.edu.cn.
⁴ School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 260 Baichuan Street, Hangzhou 311402, PR China. Electronic address: wangyiqi2011@126.com.
⁵ School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, No. 260 Baichuan Street, Hangzhou 311402, PR China. Electronic address: ybtcm@zcmu.edu.cn.

PMID: 35306017
DOI: 10.1016/j.ijbiomac.2022.03.072

Abstract

A bioactive polysaccharide (TS2-2A) with a molecular weight of 15 kDa was isolated from Trametes sanguinea Lloyd, a medicinal food homologous fungus, by water extraction-alcohol precipitation and chromatographic separation. NMR analysis of polysaccharide and MS/MS analysis of its oligosaccharide indicated that TS2-2A featured a novel straight chain with a backbone of 1,3-α-d-glucopyranose and 1,4-β-d-glucopyranose at a molar ratio of 1:4. Moreover, TS2-2A, recognized by Toll-like receptor 4 (TLR4), stimulated RAW 264.7 macrophages to release related cytokines and contributed to immune-enhancing effects. Briefly, with remarkable immune-enhancing activity and noncytotoxicity, TS2-2A was proposed to be a potential immune enhancer for supplementing drugs or functional foods.

Keywords: Activation of TLR4; Immune-enhancing activity; Polysaccharide; Structural characterization; Trametes sanguinea.

MeSH terms

Animals
Mice
Polyporaceae
Polysaccharides / chemistry
RAW 264.7 Cells
Tandem Mass Spectrometry
Toll-Like Receptor 4*
Trametes*

Substances

Polysaccharides
Tlr4 protein, mouse
Toll-Like Receptor 4

Supplementary concepts

Trametes sanguinea