Fabrication of lipase-loaded particles by coacervation with chitosan

Yan-Wei Liu; Ying Zhou; Guo-Qing Huang; Li-Ping Guo; Xiao-Dan Li; Jun-Xia Xiao

doi:10.1016/j.foodchem.2022.132689

Fabrication of lipase-loaded particles by coacervation with chitosan

Food Chem. 2022 Aug 15:385:132689. doi: 10.1016/j.foodchem.2022.132689. Epub 2022 Mar 11.

Authors

Yan-Wei Liu¹, Ying Zhou², Guo-Qing Huang², Li-Ping Guo², Xiao-Dan Li², Jun-Xia Xiao³

Affiliations

¹ College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China; College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
² College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
³ College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: xjxfood@qau.edu.cn.

PMID: 35303653
DOI: 10.1016/j.foodchem.2022.132689

Abstract

Coacervation of the lipase from Aspergillus oryzae (AOL) with chitosan was a feasible way to fabricate lipase-loaded particles and the optimum conditions were phase separation pH 5.5, chitosan to AOL mass ratio 1:5, and temperature 25 °C in the absence of NaCl, which conferred an AOL loading efficiency of up to 95.48% and activity recovery of 69.60%. The AOL-chitosan coacervates were highly porous and more susceptible to weight loss upon heating. Coacervation with chitosan increased the activity of AOL and shifted its optimum pH from 7.0 to 6.0, but exerted no effect on its optimum temperature (45 °C). Thermal deactivation kinetics analysis revealed that the coacervated AOL was more thermal stable, while the Michaelis-Menten kinetics analysis indicated that coacervation with chitosan increased the V_max of AOL by 2.4 folds, but decreased its substrate affinity by 3.6 folds. Hence, the AOL-chitosan coacervates are potential in the construction of Pickering emulsion-based lipase catalysis systems.

Keywords: Chitosan; Complex coacervation; Enzymatic kinetics; Lipase; Thermal stability.

MeSH terms

Aspergillus oryzae* / metabolism
Chitosan*
Hydrogen-Ion Concentration
Kinetics
Lipase / metabolism

Substances

Chitosan
Lipase