Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

Ana Paula Scaramal Ricietto; Kátia Cristiane Brumatti Gonçalves; Renan José Casarotto Appel; Daniel Ricardo Sosa-Gómez; Gislayne Trindade Vilas-Bôas; Laurival Antonio Vilas-Boas

doi:10.1590/1678-4685-GMB-2021-0289

Complete genome sequence of Bacillus thuringiensis BR145, a strain with insecticidal activity against Lepidoptera pests

Genet Mol Biol. 2022 Mar 16;45(2):e20210289. doi: 10.1590/1678-4685-GMB-2021-0289. eCollection 2022.

Authors

Ana Paula Scaramal Ricietto¹, Kátia Cristiane Brumatti Gonçalves², Renan José Casarotto Appel², Daniel Ricardo Sosa-Gómez³, Gislayne Trindade Vilas-Bôas², Laurival Antonio Vilas-Boas²

Affiliations

¹ Universidade Norte do Paraná, Londrina, PR, Brazil.
² Universidade Estadual de Londrina, Departamento de Biologia Geral, Londrina, PR, Brazil.
³ Empresa Brasileira de Pesquisa Agropecuária (Embrapa Soja), Centro Nacional de Pesquisa de Soja, Londrina, PR, Brazil.

Abstract

Bacillus thuringiensis BR145 isolated from a soybean field in Southern Brazil showed toxicity against two important insect pests from soybean crop, Helicoverpa armigera, and Chrysodeixis includens, with LC50 0.294 µg.cm-2 and 0.277 µg.cm-2, respectively. We analyzed the genome of this strain through sequences obtained by Next Generation DNA Sequencing and de novo assembly. The analysis of the genome revealed insecticidal genes cry1Aa, cry1Ab, cry1Ac, cry1Ia, cry2Ab, cyt1, and vip3Aa, suggesting the use of this strain in new strategies of biological control.