Gated ion channels in biological cell membranes allow efficient tuning of cross-membrane ion transport with enhanced permeation and selectivity, converting ionic signals into various forms of electrical signals and energies on demands, which functionalities though are still difficult to achieve in artificial membranes. Here, we report cation-gated ion transport through synthesized porous aromatic films containing nanometer-scale ionic channels together with -NH2 groups at interiors. Ion selectivity and permeability is greatly tuned by gating cations, up to 2 orders of magnitude, and as a consequence, the membrane efficiently produces switchable electricity output from salinity gradients. The results are attributed to positively charged cations binding at -NH2 groups, which screens the intrinsic negative surface charge at channels' interiors and inverts charge polarity there. Our work adds understanding to ion gating effects at nanoscale and offers strategies of developing smart membranes and their heterostructures for separation, energy conversion, cell membrane mimics, and related technologies.