Facile monitoring of meat freshness with a self-constructed photosensitization colorimetric instrument

Abstract

Total volatile basic nitrogen (TVB-N) produced from the decomposition of amino acids is an important indicator for meat freshness. Various pH-sensitive colorimetric films have been incorporated as intelligent packaging for meat freshness during food transportation. However, methods and instruments capable of on-site end-point detection of meat freshness are still needed for places that provide raw meat without packaging. Herein, based on amine-induced pH change that led to decreased color output of the 3,3',5,5'-tetramethylbenzidine (TMB)-based photosensitization colorimetric assay, a simple yet convenient instrument employing colorimetric indicator paper (CIP) was constructed for facile monitoring of meat freshness. Owing to the background color provided by the photosensitizer erythrosine (2',4',5',7'-tetraiodofluorescein, TIF), the color changed from blue to pink upon amine adsorption. A bespoke cellphone App was employed for image capture and color analysis of the CIP for freshness monitoring. The analytical results of amine (released from meat during storage) by the proposed method agreed well with those by a standard Conway dish method. In addition, the whole analytical process could be completed in about 5 min. The developed instrument may be potentially useful for on-site monitoring of meat freshness.

Keywords: Colorimetric indicator paper; Meat freshness; On-site monitoring; Photosensitization colorimetric assay.