An In Silico Investigation of SPC24 as a Putative Biomarker of Kidney Renal Clear Cell Carcinoma and Kidney Renal Papillary Cell Carcinoma for Predicting Prognosis and/or Immune Infiltration

Shengqiang Fu; Binbin Gong; Yi Ding; Changshui Zhuang; Qiang Chen; Siyuan Wang; Zhilong Li; Ming Ma; Yifu Liu; Zhicheng Zhang; Ting Sun

doi:10.2174/1386207325666220315105054

An In Silico Investigation of SPC24 as a Putative Biomarker of Kidney Renal Clear Cell Carcinoma and Kidney Renal Papillary Cell Carcinoma for Predicting Prognosis and/or Immune Infiltration

Comb Chem High Throughput Screen. 2022;25(13):2278-2294. doi: 10.2174/1386207325666220315105054.

Authors

Shengqiang Fu^{1

2}, Binbin Gong¹, Yi Ding^{1

2}, Changshui Zhuang³, Qiang Chen^{1

2}, Siyuan Wang^{1

2}, Zhilong Li^{1

2}, Ming Ma¹, Yifu Liu^{1

2}, Zhicheng Zhang^{1

2}, Ting Sun¹

Affiliations

¹ Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
² Jiangxi Institute of Urology, Nanchang, China.
³ Department of Urology, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, China.

PMID: 35293292
DOI: 10.2174/1386207325666220315105054

Abstract

Background and objective: SPC24 was reported to be correlated with the development of many cancers. However, its role in renal cancer was unclear. Our aim was to explore the role of SPC24 in kidney renal clear cell carcinoma (KIRC) and kidney renal papillary cell carcinoma (KIRP) in types of renal cancer.

Methods: SPC24 expressions in KIRC and KIRP were firstly analyzed. Subsequently, the correlation between SPC24 expression and TNM staging of KIRC and KIRP and the accuracy of SPC24 in diagnosing KIRC and KIRP were explored. Moreover, the correlation between SPC24 expression and prognosis of KIRC and KIRP were analyzed. Univariate and multivariate analyses were performed to identify prognostic factors in KIRC and KIRP, and nomograms were constructed. The correlation between SPC24 expression and immune cell infiltration, immune molecules, microsatellite instability (MSI), and tumor mutational burden (TMB) were further explored. Finally, the correlations between SPC24 expression and prognosis of KIRC based on different immune cell enrichment were analyzed.

Results: SPC24 was significantly up-regulated in multiple cancers, especially KIRC and KIRP. SPC24 expression was significantly correlated with the TNM stage of KIRC and KIRP, and upregulated SPC24 suggested a worse prognosis. Besides, SPC24 possesses good accuracy in diagnosing KIRC and KIRP. The SPC24-based nomograms displayed satisfactory efficacy in KIRC and KIRP. Moreover, we found that SPC24 expression was closely correlated with immune cell infiltration, immune molecules, and TMB in KIRC, and up-regulated SPC24 revealed poor prognosis based on different immune cell enrichment.

Conclusion: SPC24 has the potential to be a biomarker predicting the prognosis and/or immune infiltration of KIRC and KIRP.

Keywords: SPC24; immune infiltration; kidney renal clear cell carcinoma; kidney renal papillary cell carcinoma; prognosis; putative biomarker.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor
Carcinoma, Renal Cell* / diagnosis
Carcinoma, Renal Cell* / genetics
Humans
Kidney / metabolism
Kidney / pathology
Kidney Neoplasms* / diagnosis
Kidney Neoplasms* / genetics
Microtubule-Associated Proteins
Neoplasm Staging

Substances

Biomarkers, Tumor
Microtubule-Associated Proteins
SPC24 protein, human