Radicular canal disinfection using photodynamic therapy and sodium hypochlorite following three chelating agents' green tea extract, grape extract, and EDTA on the push-out bond strength of epoxy resin-based sealer

Abstract

Aim: To appraise the efficacy of photodynamic therapy (PDT) and conventional regime (NaOCl) following three chelating agents ethylene diamine tetraacetic acid (EDTA), Green tea extract (GTE), grape extract (GE) on pushout bond strength (PBS) of epoxy resin-based sealer with root dentin.

Material and methods: 60 single-rooted human teeth were prepped using a ProTaper system and cleansed using photodynamic therapy (PDT) and 5% NaOCl (n = 30 each). Samples in each group PDT and 5% NaOCl were further divided into 6 sub-group (n = 10) based on the chelating agents used: 17% EDTA, GTE, and GE. Gutta-percha and AH Plus were used to obturate the canals. The push-out test was used to determine bond strength, and ANOVA was used to conduct statistical analysis while failure patterns were classified as adhesive, cohesive, or mixed. The Chi-squared test was used to examine the different failure modes at a significance level of 0.05.

Results: NaOCl disinfection, when applied with naturally derived reducing agents (17% EDTA, GTE, and GE) demonstrated significantly higher PBS compared to PDT when used with chelating agents (17% EDTA, GTE, and GE) (p < 0.05). The most predominant failure mode was an adhesive failure when root dentin was disinfected with PDT while NaOCl treatment showed a high percentage of cohesive failure.

Conclusion: Radicular canal disinfection with sodium hypochlorite following three chelating agents (Green tea extract, Grape extract, and Ethylene diamine tetraacetic acid) exhibited better push-out bond strength bonded to radicular dentin with epoxy resin-based sealer.

Keywords: Ethylene diamine tetraacetic acid; Grape extract; Green tea extract; Methylene blue; Photodynamic therapy; Push out bond strength; Sodium hypochlorite; chelating agents.