One-step synthesis of glycogen-type polysaccharides from maltooctaose and its structural characteristics

Phuong Lan Tran; Yan An; Goo-Yeong Jeong; So-Young Ban; Phu Cuong Nguyen; Euijeon Woo; SangGuan You; Jong-Tae Park

doi:10.1016/j.carbpol.2022.119175

One-step synthesis of glycogen-type polysaccharides from maltooctaose and its structural characteristics

Carbohydr Polym. 2022 May 15:284:119175. doi: 10.1016/j.carbpol.2022.119175. Epub 2022 Jan 25.

Authors

Phuong Lan Tran¹, Yan An², Goo-Yeong Jeong³, So-Young Ban³, Phu Cuong Nguyen⁴, Euijeon Woo⁵, SangGuan You⁶, Jong-Tae Park⁷

Affiliations

¹ Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea; Department of Food Technology, An Giang University, An Giang 880000, Viet Nam; Vietnam National University, Ho Chi Minh City 700000, Viet Nam.
² Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea; Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju, 28644, Chungbuk, Republic of Korea.
³ Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea; CarboExpert Inc., Daejeon 34134, Republic of Korea.
⁴ Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea.
⁵ Disease Target Structure Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea; Department of Bioscience, KRIBB School, University of Science and Technology, Daejeon 34141, Republic of Korea.
⁶ East Coast Life Science Institute, Gangneung-Wonju National University, 120 Gangneung, 25457, Gangwon, Republic of Korea.
⁷ Department of Food Science and Technology, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address: jtpark@cnu.ac.kr.

PMID: 35287897
DOI: 10.1016/j.carbpol.2022.119175

Abstract

The one-step synthesis of glycogen-type polysaccharides from maltooctaose (G8) was accomplished based on the new findings of the catalytic mechanism of glycogen branching enzymes (GBEs) from Vibrio vulnificus, Deinococcus geothermalis, and Escherichia coli. GBEs from D. geothermalis and E. coli used maltononaose and maltotridecaose as the minimum, respectively, while V. vulnificus GBE (VvGBE) catalyzed the surprisingly small substrate, G8, into polysaccharides. Intriguingly, all three GBEs catalyzed α-1,4-transglycosylation at the early reaction stage of transglycosylation. VvGBE successfully converted the smallest substrate (G8) into two highly branched polysaccharides (HBP), in which the big polysaccharide (1.49 × 10⁵ Da) exhibited structural properties similar to glycogen. Both HBPs had similar side chain distribution with a very short average degree of polymerization compared with mussel glycogen. As a molecular biology reagent, these nucleotide-free HBPs significantly increased the mRNA extraction efficiency of mammalian cells. Our results provide a new approach to the synthesis of novel polysaccharides.

Keywords: Glycogen branching enzyme; Glycogen-type polysaccharide; Maltooctaose; Polysaccharide synthesis; α-1,4-Transglycosylation.