Deciphering external chain length and cyclodextrin production with starch catalyzed by cyclodextrin glycosyltransferase

Hangyan Ji; Yuxiang Bai; Yixi Liu; Yanli Wang; Xiaobei Zhan; Jie Long; Long Chen; Chao Qiu; Zhengyu Jin

doi:10.1016/j.carbpol.2022.119156

Deciphering external chain length and cyclodextrin production with starch catalyzed by cyclodextrin glycosyltransferase

Carbohydr Polym. 2022 May 15:284:119156. doi: 10.1016/j.carbpol.2022.119156. Epub 2022 Jan 20.

Authors

Hangyan Ji¹, Yuxiang Bai¹, Yixi Liu², Yanli Wang², Xiaobei Zhan³, Jie Long¹, Long Chen¹, Chao Qiu¹, Zhengyu Jin⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
³ School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province, China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, Jiangsu Province, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, Jiangsu Province, China. Electronic address: fpcenter@jiangnan.edu.cn.

PMID: 35287893
DOI: 10.1016/j.carbpol.2022.119156

Abstract

Cyclodextrins (CDs) are yielded by cyclodextrin glycosyltransferase (CGTase) using starches or their derivatives as substrates. However, how starch fine structure affects it production is still ambiguous. This study aimed to decipher the relevance between the external chain length (ECL) and CD production. The waxy maize starch was hydrolyzed by β-amylase to prepare starchy substrates with regular gradient ECL for CGTase. The HPAEC results reflected that β-amylolysis lower the starch chain fractions with DP range of 13-24 by 30%, while the fractions with DP < 6 promoted by 30%. The molecular weight distribution and iodine binding results reflected that this process had limited impact on overall starch molecular size parameters. After CGTase conversion, it is interesting to find that the ECL was positively correlated with CD contents, which also has a high consistency among α-CD, β-CD, and γ-CD. This research would provide a favorable perspective for CD production improvement.

Keywords: Cyclodextrin glycosyltransferase; Cyclodextrin production; External chain length; Structural characterization; Waxy maize starch.

MeSH terms

Catalysis
Cyclodextrins* / metabolism
Glucosyltransferases / metabolism
Starch / chemistry
Substrate Specificity

Substances

Cyclodextrins
Starch
Glucosyltransferases
cyclomaltodextrin glucanotransferase