Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori

Guanfeng Xu; Yulin Tian; Yuling Peng; Sichun Zheng

doi:10.1016/j.xpro.2022.101219

Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori

STAR Protoc. 2022 Mar 7;3(1):101219. doi: 10.1016/j.xpro.2022.101219. eCollection 2022 Mar 18.

Authors

Guanfeng Xu^{1

2}, Yulin Tian^{1

2}, Yuling Peng^{1

2}, Sichun Zheng^{1

2}

Affiliations

¹ Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
² Guangzhou Key Laboratory of Insect Development Regulation and Applied Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.

Abstract

RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-stranded RNAs (dsRNAs) of target genes, followed by microinjection of embryos at different developmental stages, with single or mixed dsRNA. Finally, we use RT-qPCR to verify RNAi efficiency. For complete details on the use and execution of this protocol, please refer to Xu et al. (2021).

Keywords: Developmental biology; Gene Expression; Genetics; Model Organisms; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bombyx* / genetics
Insecta / genetics
RNA Interference
RNA, Double-Stranded / genetics

Substances

RNA, Double-Stranded