Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis

Yifan Gao; Kshitjia Shah; Ivy Kwok; Meng Wang; Leonard H Rome; Shaily Mahendra

doi:10.1016/j.biotechadv.2022.107936

Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis

Biotechnol Adv. 2022 Jul-Aug:57:107936. doi: 10.1016/j.biotechadv.2022.107936. Epub 2022 Mar 8.

Authors

Yifan Gao¹, Kshitjia Shah¹, Ivy Kwok¹, Meng Wang², Leonard H Rome³, Shaily Mahendra⁴

Affiliations

¹ Department of Civil and Environmental Engineering, University of California, Los Angeles, CA 90095, United States.
² Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA 15260, United States.
³ Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA 90095, United States.
⁴ Department of Civil and Environmental Engineering, University of California, Los Angeles, CA 90095, United States; California NanoSystems Institute, University of California, Los Angeles, CA 90095, United States. Electronic address: mahendra@seas.ucla.edu.

PMID: 35276253
DOI: 10.1016/j.biotechadv.2022.107936

Abstract

Microbial enzymes catalyze various reactions inside and outside living cells. Among the widely studied enzymes, fungal enzymes have been used for some of the most diverse purposes, especially in bioremediation, biosynthesis, and many nature-inspired commercial applications. To improve their stability and catalytic ability, fungal enzymes are often immobilized on assorted materials, conventional as well as nanoscale. Recent advances in fungal enzyme immobilization provide effective and sustainable approaches to achieve improved environmental and commercial outcomes. This review aims to provide a comprehensive overview of commonly studied fungal enzymes and immobilization technologies. It also summarizes recent advances involving immobilized fungal enzymes for the degradation or assembly of compounds used in the manufacture of products, such as detergents, food additives, and fossil fuel alternatives. Furthermore, challenges and future directions are highlighted to offer new perspectives on improving existing technologies and addressing unexplored fields of applications.

Keywords: Biocatalyst; Bioengineering; Biotransformation; Encapsulation; Synthesis.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis
Biodegradation, Environmental
Enzymes, Immobilized* / metabolism

Substances

Enzymes, Immobilized