Effects of polyethylene, polyvinyl chloride, and polystyrene microplastics on the vermitoxicity of fluoranthene in soil

Abstract

Polycyclic aromatic hydrocarbons (PAHs) and microplastics (MPs) may coexist in soil. Consequently, the toxicity of PAHs to "soil engineers" earthworms (vermitoxicity), may be influenced by various MPs. However, studies on this issue are scarce. In particular, the differential effect of MPs of different polymers on the vermitoxicity of PAHs remains unclear. Therefore, a series of microcosm experiments were conducted to evaluate the potential vermitoxicity of fluoranthene (Fla) in the presence of polyethylene (PE), polyvinyl chloride (PVC), and polystyrene (PS) MPs at an environmentally relevant concentration (125 mg kg^-1). The results indicated that Fla exerted vermitoxicity and MPs enhanced the adverse effects. Specifically, after the introduction of MPs, the 14-d LC₅₀s of Fla on earthworms decreased from 130.9 to 98.0-123.6 mg kg^-1; in addition, the growth inhibition rates improved from 5.37%-15.34% to 15.63%-33.38%, and the avoidance rate increased by 10%-100% at the same exposure doses in most cases. In most cases, the neutral red retention time was shortened by 14.3%-47.9%, indicating that the integrity of the coelomocytes' lysosomal membrane had worsened. The affected antioxidant enzyme activity and improved malondialdehyde content indicated enhanced oxidative damage in the treatments containing MPs. However, the aggravation of the vermitoxicity varied by MPs type and toxicological endpoints. Overall, Fla + PS MPs exerted the greatest effect on the mortality of earthworms. On the contrary, PVC and PE MPs exhibited higher subacute effects on the vermitoxicity of Fla. Our study also demonstrated that MPs at environmentally relevant doses may directly induce vermitoxicity. In particular, damaged coelomocytes' lysosomal membrane stability by MPs was second reported to the best of our knowledge. Our results revealed the differences in the effects of various MPs on the vermitoxicity of PAHs, which provides new data in assessing the ecological effects of PAHs and MPs in soils.

Keywords: Acute toxicity; Antioxidant enzyme; Earthworm; Microplastic; Neutral red retention time (NRRT); Polycyclic aromatic hydrocarbons (PAHs).