Upregulation of PODXL and ITGB1 in pancreatic cancer tissues preoperatively obtained by EUS-FNAB correlates with unfavorable prognosis of postoperative pancreatic cancer patients

Keisuke Taniuchi; Makoto Ueno; Tomoyuki Yokose; Masahiko Sakaguchi; Reiko Yoshioka; Mitsunari Ogasawara; Takuhiro Kosaki; Seiji Naganuma; Mutsuo Furihata

doi:10.1371/journal.pone.0265172

Upregulation of PODXL and ITGB1 in pancreatic cancer tissues preoperatively obtained by EUS-FNAB correlates with unfavorable prognosis of postoperative pancreatic cancer patients

PLoS One. 2022 Mar 11;17(3):e0265172. doi: 10.1371/journal.pone.0265172. eCollection 2022.

Authors

Keisuke Taniuchi¹, Makoto Ueno², Tomoyuki Yokose³, Masahiko Sakaguchi⁴, Reiko Yoshioka¹, Mitsunari Ogasawara¹, Takuhiro Kosaki⁵, Seiji Naganuma⁶, Mutsuo Furihata⁶

Affiliations

¹ Department of Gastroenterology and Hepatology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.
² Department of Gastroenterology, Hepatobiliary and Pancreatic Medical Oncology Division, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
³ Department of Pathology, Kanagawa Cancer Center, Yokohama, Kanagawa, Japan.
⁴ Faculty of Information and Communication Engineering, Osaka Electro-Communication University, Osaka, Japan.
⁵ Department of Endoscopic Diagnostics and Therapeutics, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.
⁶ Department of Pathology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.

Abstract

The upregulation of PODXL and ITGB1 in surgically resected pancreatic cancer tissues is correlated with an unfavorable postoperative prognosis. The aim of this study was to investigate whether PODXL and ITGB1 are useful preoperative markers for the prognosis of postoperative pancreatic cancer patients in comparison with the TNM staging system. Immunohistochemistry was performed using anti-PODXL and anti-ITGB1 antibodies on 24 pancreatic cancer tissue samples preoperatively obtained by endoscopic ultrasound-guided fine-needle aspiration biopsy. Cox proportional hazards regression analysis was performed to investigate if the UICC TNM stage and upregulation of PODXL and ITGB1 were correlated with postoperative overall survival rates. Univariate analysis revealed that PODXL, TNM stage, lymphatic invasion and the combination of PODXL with ITGB1 are correlated with postoperative survival. Multivariate analysis demonstrated TNM stage and the combination of PODXL with ITGB1 to be correlated with postoperative survival, and the combination of PODXL with ITGB1 most accurately predicted the postoperative outcomes of pancreatic cancer patients before resection. Therefore, upregulation of PODXL and ITGB1 may indicate preoperative neoadjuvant therapy for pancreatic cancer patients by accurately predicting the postoperative prognosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Endoscopic Ultrasound-Guided Fine Needle Aspiration*
Humans
Pancreatic Neoplasms* / diagnostic imaging
Pancreatic Neoplasms* / surgery
Prognosis
Up-Regulation

Grants and funding

This study was supported by Grants-in-Aid for Scientific Research (KAKENHI): 17K09463 (kt), 19K07461 (mf), 20K07806 (ry), 20K08359 (kt) and 20K07699 (mo), and AMED under Grant Number JP19lm0203007 (kt). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.