Gradient hydrogels are promising future materials which could be usable in tissue engineering (scaffolds), pharmaceutical (drug delivery systems with controlled release) and many others related disciplines. These hydrogels exhibit a more complex inner (gradient) structure (e.g., concentration gradient) than simple isotropic hydrogel. Gradient-structured hydrogels could be beneficial in, for example, understanding intercellular interactions. The fabrication of gradient hydrogels has been relatively deeply explored, but a comprehensive description of the physico-chemical techniques demonstrating the existence of a gradient structure is still missing. Here, we summarize the state-of-the-art available experimental techniques applicable in proving and/or describing in physico-chemical terms the inner gradient structure of hydrogels. The aim of this paper is to give the reader an overview of the existing database of suitable techniques for characterizing gradient hydrogels.
Keywords: biopolymer 3D gels; concentration gradient; gradient hydrogels; intelligent supramocelular gels; physico-chemical techniques for gradient proof.