Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology excels at long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.
Keywords: DNA barcoding; Oxford Nanopore Technologies; amplicon sequencing; biodiversity; consensus; metabarcoding; metagenetics; replacing Sanger.
© 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.