Purpose: To compare perinatal outcomes of singletons after blastocyst versus cleavage-stage embryo transfer in frozen-thawed embryo transfer (FET) cycles.
Methods: This was a retrospective cohort study of 4835 women who conceived after frozen-thawed embryo transfer and delivered a singleton baby from January 2015 to March 2020. The perinatal outcomes of the singletons born after blastocyst transfer (N = 3364) were compared to the singletons born after cleavage-stage transfer (N = 1471), including gestational week, birth weight, newborn gender and Apgar score.
Results: Univariate and multivariate analysis found that infants born after blastocyst transfer had a higher risk of preterm birth (PTB) [adjusted odds ratio (aOR) 1.480; 95% confidence interval (CI) 1.213-1.807], large for gestational age (LGA) (aOR 1.329, 95%CI 1.149-1.536) and very large for gestational age (VLGA) (aOR 1.317, 95%CI 1.092-1.590) compared to infants born after cleavage-stage embryo transfer. When categorized by gestational weeks, the proportion of LGA was significantly higher for boys born after BT between GW 37 to 41 (aOR 1.301, 95%CI 1.051-1.609) and for girls born after BT between GW 32 to 41 (aOR 2.690, 95%CI 1.154-6.271, aOR 1.377, 95%CI 1.094-1.733), compared to boys and girls born after CT.
Conclusion: Our data suggested that the risk of PTB and LGA increased after blastocyst transfer versus cleavage-stage embryo transfer in FET cycles, and the risk of LGA is more apparent in the last trimester of pregnancy. Further studies are needed to confirm our findings and explored the association between the extended embryo culture and long-term outcomes of offspring.
Keywords: Blastocyst; Cleavage-stage embryo; Frozen-thawed embryo transfer; Large for gestational age; Preterm birth.
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