The Fusarium toxins constitute one of the largest groups of mycotoxins produced by Fusarium species, which are major pathogens of cereal plants. In the present study neuroprotection effect of Allium sativum L garlic extract which is known as Voghiera garlic, from a local garlic ecotype of Ferrara (Italy) was examined on an undifferentiated SH-SY5Y neuronal cells against ZEA's metabolites (α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL)) and beauvericin (BEA) mycotoxins which are considered as the most reported Fusarium mycotoxins, via MTT (3-4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, over 24 h and 48 h through direct treatment, simultaneous treatment and pre-treatment strategies. The results demonstrated remarkable improvement in cells viability in simultaneous and pre-treatment strategy with Voghiera garlic extract (VGE); specifically, for simultaneous treatment of VGE with β-ZEL which viability increased significantly up to 56%, and subsequently with α-ZEL and BEA by up to 38% and 37% respectively, compared to each mycotoxin tested alone for their highest concentrations assayed, while direct treatments for each mycotoxins individually decreased significantly (for α-ZEL up to 69%, for β-ZEL 82% and for BEA up to 43%). It is proposed by the present study that VGE extract found to be effective in reducing the cytotoxicity/neurotoxicity of α-ZEL, β-ZEL and BEA mycotoxins encountered in food and feed commodity.
Keywords: BEA; SH-SY5Y cells; Voghiera garlic; α-ZEL; β-ZEL.
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