A stable enzyme sensor via embedding enzymes into zeolitic imidazolate frameworks for pesticide determination

Yisong Zhao; Xiong Lu; Faming Gao

doi:10.1016/j.ab.2022.114628

A stable enzyme sensor via embedding enzymes into zeolitic imidazolate frameworks for pesticide determination

Anal Biochem. 2022 Jun 1:646:114628. doi: 10.1016/j.ab.2022.114628. Epub 2022 Mar 4.

Authors

Yisong Zhao¹, Xiong Lu¹, Faming Gao²

Affiliations

¹ Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.
² Key Laboratory of Applied Chemistry, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China. Electronic address: fmgao@ysu.edu.cn.

PMID: 35257680
DOI: 10.1016/j.ab.2022.114628

Abstract

The stability of biosensors is of significant importance for practical applications, and the natural biomineralization processes in living organisms have inspired us from a new perspective. In this work, acetylcholinesterase (AChE) was embedded into zeolitic imidazolate framework-8 carriers (with negligible cytotoxicity) via biomimetic mineralization, being demonstrated to be a stable strategy for enzyme immobilization. When further coupled with the conductive and catalytic Au nanoparticles, the biocomposites were explored as electrochemical pesticide detection biosensor, which showed favorable analytical performance, and improved stability comparing with other biosensors. This work provides a new strategy for the reasonable design of stable biosensors for different analytes monitoring.

Keywords: Biomimetic mineralization; Pesticide determination; Stable electrochemical biosensor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylcholinesterase / chemistry
Biosensing Techniques*
Enzymes, Immobilized / chemistry
Gold / chemistry
Metal Nanoparticles* / chemistry
Pesticides* / analysis
Zeolites* / chemistry

Substances

Enzymes, Immobilized
Pesticides
Zeolites
Gold
Acetylcholinesterase