Functional antibody-dependent cell mediated cytotoxicity (ADCC) responses to vaccine and circulating influenza strains following vaccination

Xuemin Chen; He-Ying Sun; Chun Yi Lee; Christina A Rostad; Jessica Trost; Rodrigo B Abreu; Michael A Carlock; Jason R Wilson; Shane Gansebom; Ted M Ross; David A Steinhauer; Evan J Anderson; Larry J Anderson

doi:10.1016/j.virol.2022.02.004

Functional antibody-dependent cell mediated cytotoxicity (ADCC) responses to vaccine and circulating influenza strains following vaccination

Virology. 2022 Apr:569:44-55. doi: 10.1016/j.virol.2022.02.004. Epub 2022 Feb 24.

Authors

Affiliations

¹ Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States; Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta, Atlanta, GA, United States; Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Atlanta, GA, USA.
² Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States; Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Atlanta, GA, USA.
³ Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States.
⁴ Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States; Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta, Atlanta, GA, United States.
⁵ Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Atlanta, GA, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, United States; Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.
⁶ Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States; Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Athens, GA, USA.
⁷ Molecular Virology and Vaccine Team, Influenza and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Disease, OID, Centers for Disease Control and Prevention, Atlanta, GA, United States.
⁸ Molecular Virology and Vaccine Team, Influenza and Pathogenesis Branch, Influenza Division, National Center for Immunization and Respiratory Disease, OID, Centers for Disease Control and Prevention, Atlanta, GA, United States; (CDC/DDID/NCIRD/ID) GDIT, Federal Civilian Division, 2 Corporate Square; Ste 100, Atlanta, GA, 30329, USA.
⁹ Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States; Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Athens, GA, USA; Department of Infectious Diseases, University of Georgia, Athens, GA, United States.
¹⁰ Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Atlanta, GA, USA; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, United States.
¹¹ Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States; Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta, Atlanta, GA, United States; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, United States.
¹² Division of Infectious Diseases, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, United States; Center for Childhood Infections and Vaccines, Children's Healthcare of Atlanta, Atlanta, GA, United States; Emory-UGA Center of Excellence of Influenza Research and Surveillance (CEIRS), Atlanta, GA, USA. Electronic address: larry.anderson@emory.edu.

Abstract

Novel cell-based assays were developed to assess antibody-dependence cellular cytotoxicity (ADCC) antibodies against both vaccine and a representative circulation strain HA and NA proteins for the 2014-15 influenza season. The four assays using target cells stably expressing one of the four proteins worked well. In pre- and post-vaccine sera from 70 participants in a pre-season vaccine trial, we found ADCC antibodies and a rise in ADCC antibody titer against target cells expressing the 4 proteins but a much higher titer for the vaccine than the circulating HA in both pre-and post-vaccine sera. These differences in HA ADCC antibodies were not reflected in differences in HA binding antibodies. Our observations suggested that relatively minor changes on the subtype HA can result in large differences in ADCC activity.

Keywords: Antibody-dependent cellular cytotoxicity (ADCC); Influenza hemagglutinin (HA); Influenza neuraminidase (NA); Influenza virus vaccine.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Antibodies, Viral
Antibody-Dependent Cell Cytotoxicity
Cross Reactions
Hemagglutinin Glycoproteins, Influenza Virus
Humans
Influenza A Virus, H1N1 Subtype*
Influenza Vaccines*
Influenza, Human* / prevention & control
Vaccination

Substances

Antibodies, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Influenza Vaccines

Grants and funding

HHSN272201400004C/AI/NIAID NIH HHS/United States