Metabarcoding data of prokaryotes and eukaryotes inhabiting the phosphogypsum stockpiles on the salt marshes of Huelva (SW Spain)

Patricia Gómez-Villegas; José Luis Guerrero; Miguel Pérez-Rodríguez; Juan Pedro Bolivar; Javier Vigara; Rosa León

doi:10.1016/j.dib.2022.107989

Metabarcoding data of prokaryotes and eukaryotes inhabiting the phosphogypsum stockpiles on the salt marshes of Huelva (SW Spain)

Data Brief. 2022 Feb 22:41:107989. doi: 10.1016/j.dib.2022.107989. eCollection 2022 Apr.

Authors

Patricia Gómez-Villegas¹, José Luis Guerrero², Miguel Pérez-Rodríguez³, Juan Pedro Bolivar², Javier Vigara¹, Rosa León¹

Affiliations

¹ Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, Huelva 21071, Spain.
² Department of Integrated Sciences, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, Avda. de las Fuerzas Armadas s/n, Huelva 21071, Spain.
³ Department of Cell Biology, Physiology and Immunology, University of Córdoba, Campus de Excelencia Internacional Agroalimentario CeiA3, Córdoba, Spain.

Abstract

Around 100 Mt of phosphogypsum (PG) of extreme acidity and with high concentrations of heavy metals and radionuclides have been deposited on the salt marshes of the Tinto River estuary in Huelva (SW Spain) for more than forty years. The microbial community able to thrive in these adverse conditions remains totally unknown, despite the fact that it can highly influence the biogeochemical cycle of the phosphogypsum components and include new species with biotechnological interest. High throughput sequencing of 16S/18S rRNA encoding genes is a potent tool to uncover the microbial diversity of extreme environments. This data article describes for the first time the prokaryotic and eukaryotic diversity of two water samples collected in the Huelva phosphogypsum stacks. The raw amplicons of the 16S/18S rRNA maker genes for the two phosphogypsum samples and two reference samples (seawater and the Tinto River water) obtained after sequencing on MiSeq platform are provided. The operational taxonomic units (OTUs) obtained after the treatment and clustering of the obtained reads with the QIIME2 pipeline and their taxonomic assignation performed by comparison with the SILVA database are also presented to complete the information of the article "Exploring the microbial community inhabiting the phosphogypsum stacks of Huelva (SW, Spain) by a high throughput 16S/18S rDNA Sequencing approach".

Keywords: 16s rRNA; 18s rRNA; Extreme environment; Metabarcoding; Metataxonomy; Phosphogypsum.