A call for consensus guidelines on monitoring the integrity of nuclear and mitochondrial genomes in human pluripotent stem cells

Andrea Rossi; Selene Lickfett; Soraia Martins; Alessandro Prigione

doi:10.1016/j.stemcr.2022.01.019

A call for consensus guidelines on monitoring the integrity of nuclear and mitochondrial genomes in human pluripotent stem cells

Stem Cell Reports. 2022 Apr 12;17(4):707-710. doi: 10.1016/j.stemcr.2022.01.019. Epub 2022 Mar 3.

Authors

Andrea Rossi¹, Selene Lickfett², Soraia Martins³, Alessandro Prigione⁴

Affiliations

¹ Genome Engineering and Model Development Lab (GEMD), IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf 40225, Germany. Electronic address: andrea.rossi@IUF-Duesseldorf.de.
² Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf Germany; Department of Anatomy, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany.
³ Genome Engineering and Model Development Lab (GEMD), IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf 40225, Germany.
⁴ Department of General Pediatrics, Neonatology, and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf Germany. Electronic address: alessandro.prigione@hhu.de.

Abstract

Despite the widespread use of human pluripotent stem cells (hPSCs), there is no general consensus with respect to monitoring the integrity of nuclear and mitochondrial genome of hPSCs. We argue that clear guidelines should be in place, and we have identified frequently asked questions that can be used to develop a practical guide for hPSCs users.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
Genome, Mitochondrial*
Humans
Pluripotent Stem Cells*