A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection

Laïla Giordano; Valérie Allasia; Alexandra Cremades; Sophie Hok; Franck Panabières; Béatrice Bailly-Maître; Harald Keller

doi:10.1016/j.isci.2022.103877

A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection

iScience. 2022 Feb 5;25(3):103877. doi: 10.1016/j.isci.2022.103877. eCollection 2022 Mar 18.

Authors

Laïla Giordano¹, Valérie Allasia¹, Alexandra Cremades¹, Sophie Hok¹, Franck Panabières¹, Béatrice Bailly-Maître², Harald Keller¹

Affiliations

¹ Université Côte d'Azur, INRAE, CNRS, UMR1355-7254, ISA, 06903 Sophia Antipolis, France.
² Université Côte d'Azur, INSERM, U1065, C3M, 06200 Nice, France.

Abstract

Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are constituents of many membrane-bound receptors. In Arabidopsis thaliana, the MLD-containing receptor IOS1 promotes successful infection by filamentous plant pathogens. We show that the MLD of its exodomain retains IOS1 in the ER of plant cells and attenuates the infection-induced UPR. Expression of the MLD in the ios1-1 knockout background is sufficient to complement infection-related phenotypes of the mutant, such as increased UPR and reduced disease susceptibility. IOS1 interacts with the ER membrane-associated ribophorin HAP6 from the OST complex, and hap6 mutants show decreased pathogen-responsive UPR and increased disease susceptibility. Altogether, this study revealed a previously uncharacterized role of a plant receptor domain in the regulation of ER stress during infection.

Keywords: Molecular biology; Plant biology; Plant pathology.