The data of an all-solid-state acupuncture needle based potentiometric microelectrode for in vivo monitoring of calcium ions in rat cerebrospinal fluid

Jiali Zhai; Yaqun Zhang; Dongmei Zhao; Lijuan Kou; Guangtao Zhao

doi:10.1016/j.dib.2022.107949

The data of an all-solid-state acupuncture needle based potentiometric microelectrode for in vivo monitoring of calcium ions in rat cerebrospinal fluid

Data Brief. 2022 Feb 15:41:107949. doi: 10.1016/j.dib.2022.107949. eCollection 2022 Apr.

Authors

Jiali Zhai¹, Yaqun Zhang², Dongmei Zhao², Lijuan Kou³, Guangtao Zhao²

Affiliations

¹ School of Rehabilitation, Binzhou Medical University, Yantai 264003, PR China.
² School of Basic Medicine, Binzhou Medical University, Yantai 264003, PR China.
³ School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China.

Abstract

These data contain the details of the fabrication of the calcium ion-selective microelectrode (Ca²⁺-ISμEs) modified with poly(3,4-ethylenedioxythiophene)-poly(sodium 4-styrenesulfonate) (PEDOT(PSS)) as solid contact. The electrochemical impedance spectroscopy was carried out for the investigation of the resistance of the Ca²⁺-ISμEs. The thickness of the solid contact and the calcium ion-selective membrane was investigated by SEM. Potential-time curve of the electrodeposition of the PEDOT/PSS film onto the surface of the microelectrodes under the applied current of 0.5 μA for 200 s was recorded. The proposed Ca²⁺-ISμE was optimized through conditioning in different CaCl₂ solutions ranged from 1.0 × 10^-6 to 3.1 × 10^-3 M for different time before use. The anti-fouling property of the Ca²⁺-ISμEs against proteins was investigated through taking BSA as the model protein. The developed Ca²⁺-ISμEs were used for the in vivo monitoring of the calcium ions in rat cerebrospinal fluid under the stimuli of the spinal cord transection in a living animal.

Keywords: Acupuncture needle; Calcium; Potentiometric microelectrodes; Spinal cord transection.