Ultrasound treatment enhanced the ability of the porcine myofibrillar protein to bind furan compounds: Investigation of underlying mechanisms

Xiaocao Zhao; Jun Qi; Chaoxia Fan; Bo Wang; Cong Yang; Dengyong Liu

doi:10.1016/j.foodchem.2022.132472

Ultrasound treatment enhanced the ability of the porcine myofibrillar protein to bind furan compounds: Investigation of underlying mechanisms

Food Chem. 2022 Aug 1:384:132472. doi: 10.1016/j.foodchem.2022.132472. Epub 2022 Feb 16.

Authors

Xiaocao Zhao¹, Jun Qi², Chaoxia Fan¹, Bo Wang¹, Cong Yang¹, Dengyong Liu³

Affiliations

¹ College of Food Science and Technology, Bohai University, Jinzhou 121013, China.
² College of Food Science and Technology, Bohai University, Jinzhou 121013, China; Anhui Engineering Laboratory for Agro-products Processing, College of Tea & Food Science and Technology, Anhui Agricultural University, Hefei 230036, China.
³ College of Food Science and Technology, Bohai University, Jinzhou 121013, China; Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing 210095, China. Electronic address: bhdxrpyjs@126.com.

PMID: 35240573
DOI: 10.1016/j.foodchem.2022.132472

Abstract

This study was designed to explore the effects of different ultrasound power levels (0-600 W) on the ability of myofibrillar protein (MP) to bind furan compounds by analyzing the results of SDS-PAGE, particle size, Raman spectra, fluorescence intensity, solubility, turbidity, zeta potential, surface hydrophobicity, sulfhydryl content, solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS). As ultrasound power levels were increased from 0 to 500 W, the hydrophobic bonding sites, hydrogen-bonding sites, and electrostatic effects increased due to the unfolding and depolymerization of MP, thus enhancing the ability of MP to bind furan (flavor-enhancing) compounds. Consistent with these results, the positive effect of ultrasound resulted in ability of MP to bind furan compounds increased by 19.00 % to 33.32 %. However, after 600-W ultrasound treatment, the MP aggregated again and the bonding sites were re-embedded, which decreased the furan-binding ability.

Keywords: Binding ability; Furan compounds; Myofibrillar protein; Ultrasound.

MeSH terms

Animals
Furans* / analysis
Gas Chromatography-Mass Spectrometry / methods
Hydrophobic and Hydrophilic Interactions
Solid Phase Microextraction* / methods
Swine

Substances

Furans
furan