Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis

Keman Xu; Fatma Saaoud; Stephanie Yu; Charles Drummer 4th; Ying Shao; Yu Sun; Yifan Lu; Jianxin Sun; Jun Yu; Xiaohua Jiang; Hong Wang; Xiaofeng Yang

doi:10.1007/978-1-0716-1924-7_10

Monocyte Adhesion Assays for Detecting Endothelial Cell Activation in Vascular Inflammation and Atherosclerosis

Methods Mol Biol. 2022:2419:169-182. doi: 10.1007/978-1-0716-1924-7_10.

Authors

Keman Xu¹, Fatma Saaoud^#¹, Stephanie Yu^#², Charles Drummer 4th¹, Ying Shao¹, Yu Sun¹, Yifan Lu¹, Jianxin Sun³, Jun Yu⁴, Xiaohua Jiang^{1

4}, Hong Wang⁴, Xiaofeng Yang^{5

6

7}

Affiliations

¹ Cardiovascular Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA.
² Northeastern University, Boston, MA, USA.
³ Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
⁴ Metabolic Disease Research, Departments of Pharmacology, Microbiology and Immunology, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA.
⁵ Cardiovascular Research Center, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA. xfyang@temple.edu.
⁶ Metabolic Disease Research, Departments of Pharmacology, Microbiology and Immunology, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA. xfyang@temple.edu.
⁷ Centers for Cardiovascular Research, Inflammation & Lung Research, Metabolic Disease Research, and Thrombosis Research, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA. xfyang@temple.edu.

^# Contributed equally.

Abstract

Monocyte adhesion assay, a fluorescence-based method, enables the detection and quantification of monocyte adhesion to endothelial cell (EC) monolayers in vitro and measures EC activation. We describe in this chapter a monocyte adhesion assay based on two published papers from our laboratory that can be effectively used in studying the mechanisms of both pro- and anti-inflammatory cytokines in EC activation. Endothelial cell monolayers are cultured and treated with desired drug, cytokines, or other stimuli and incubated with fluorescently labeled monocytes.

Keywords: Adhesion molecules; Atherosclerosis; Endothelial cell activation; Inflammation; Monocyte adhesion assay; Monocytes.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Atherosclerosis* / metabolism
Cell Adhesion
Cells, Cultured
Endothelial Cells
Endothelium, Vascular / metabolism
Humans
Inflammation / metabolism
Monocytes* / metabolism

Abstract

Publication types

MeSH terms

Grants and funding