mNG-tagged mls-2 knock-in alleles in C. elegans

Rui Xiong; Yi-Wen Hsieh; Chiou-Fen Chuang

doi:10.17912/micropub.biology.000529

mNG-tagged mls-2 knock-in alleles in C. elegans

MicroPubl Biol. 2022 Feb 22:2022:10.17912/micropub.biology.000529. doi: 10.17912/micropub.biology.000529. eCollection 2022.

Authors

Rui Xiong¹, Yi-Wen Hsieh¹, Chiou-Fen Chuang^{1

2}

Affiliations

¹ Department of Biological Sciences, University of Illinois at Chicago.
² Graduate Program in Neuroscience, University of Illinois at Chicago.

Abstract

The Caenorhabditis elegans HMX/NKX MLS-2 transcription factor was previously shown to play sequential roles in AWC general identity and the stochastic AWC^ON/AWC^OFF subtype choice during embryogenesis. Here we analyze the expression pattern of endogenous mls-2 during AWC development using mNeonGreen (mNG) knock-in strains. Similar to transgenic GFP::MLS-2, functional mNG::MLS-2 knock-in displayed nuclear localization in AWC precursor cells but was not observed in AWC during the later embryonic stage. These results suggest that the expression of mls-2 is below the detectable level and/or the stability of MLS-2 protein is very low in AWC cells.