Preparation and evaluation of ultra-long open-tubular capillary columns modified with zeolitic imidazolate framework-8 incorporated polymeric porous layer for liquid chromatography

Zhihua Zhong; Manman Zhu; Zhanying Chu; Xingfa Ren; Bingfang Tu; Weibing Zhang; Lingyi Zhang

doi:10.1016/j.chroma.2022.462880

Preparation and evaluation of ultra-long open-tubular capillary columns modified with zeolitic imidazolate framework-8 incorporated polymeric porous layer for liquid chromatography

J Chromatogr A. 2022 Apr 12:1668:462880. doi: 10.1016/j.chroma.2022.462880. Epub 2022 Feb 4.

Authors

Zhihua Zhong¹, Manman Zhu², Zhanying Chu³, Xingfa Ren⁴, Bingfang Tu⁵, Weibing Zhang⁶, Lingyi Zhang⁷

Affiliations

¹ Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
² Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; Center for Advanced Measurement Science, Technology Innovation Center of Mass Spectrometry for State Market Regulation, National Institute of Metrology, Beijing 100029, China.
³ Center for Advanced Measurement Science, Technology Innovation Center of Mass Spectrometry for State Market Regulation, National Institute of Metrology, Beijing 100029, China.
⁴ Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; Welch Materials, Inc. Shanghai 200237, China.
⁵ Welch Materials, Inc. Shanghai 200237, China.
⁶ Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: weibingzhang@ecust.edu.cn.
⁷ Shanghai Key Laboratory of Functional Materials Chemistry, Department of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address: zhanglingyi@ecust.edu.cn.

PMID: 35220014
DOI: 10.1016/j.chroma.2022.462880

Abstract

An ultra-long (5 m) open tubular capillary liquid chromatographic column was prepared by incorporating Metal Organic Framework (MOF), zeolitic imidazolate framework-8 (ZIF-8), directly into polymer coating, which was synthesized by the copolymerization of 4-vinylbenzyl chloride and divinylbenzene, on the capillary inner surface. The prepared ZIF-8 incorporate polymeric open tubular capillary column (denoted as ZIF-8-p(VBC/DVB) OTCC) was evaluated with thiourea, alkylbenzenes and polycyclic aromatic hydrocarbons as probe molecules. The results showed that the ultra-long column achieved absolute column efficiency of 130,000 plates for thiourea, and the incorporation of ZIF-8 effectively improved the chromatography performance of the OTCC. Baseline separation of aromatic compounds and position isomers was achieved based on multiple interactions provided by the zeolitic imidazolate framework and polymer, including hydrophobic interaction, π-π stacking interaction and the coordination effect. The RSD values (run-to-run, day-to-day, column-to-column, n = 3) of retention time of phenylenediamine isomers and propylbenzene isomers were less than 0.7%, 1.2% and 4.0% respectively, suggesting excellent repeatability. Finally, the prepared ZIF-8-p(VBC/DVB) OTCC was applied to the separation of hydroxyacetophenone isomers with satisfied results.

Keywords: Liquid chromatography; Metal organic framework; Open-tubular capillary column.

MeSH terms

Capillary Electrochromatography* / methods
Chromatography, Liquid
Polymers / chemistry
Porosity
Zeolites* / chemistry

Substances

Polymers
Zeolites