DNA microcircles - The promising tool for in vivo studies of the behavior of non-canonical DNA

Alexandre A Vetcher; Yaroslav M Stanishevskiy

doi:10.1016/j.pbiomolbio.2022.02.002

DNA microcircles - The promising tool for in vivo studies of the behavior of non-canonical DNA

Prog Biophys Mol Biol. 2022 Mar-May:169-170:84-88. doi: 10.1016/j.pbiomolbio.2022.02.002. Epub 2022 Feb 24.

Authors

Alexandre A Vetcher¹, Yaroslav M Stanishevskiy²

Affiliations

¹ IBCTN of the Peoples' Friendship University of Russia (RUDN), 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation; Complementary and Integrative Health Clinic of Dr. Shishonin, 5 Yasnogorskaya Str, Moscow, 117588, Russian Federation. Electronic address: avetcher@gmail.com.
² IBCTN of the Peoples' Friendship University of Russia (RUDN), 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation.

PMID: 35219771
DOI: 10.1016/j.pbiomolbio.2022.02.002

Abstract

The paper discusses the reasons for the resurrection of the term DNA microcircles through the change of its definition to "topologically closed DNA circles with the length less than 1 Kbp" from the entire population of circular DNA that holds the name of minicircles. The possible applications of such tool for in vivo studies of non-canonical DNA are also discussed. Prospective for in vivo and in vitro studies of non-canonical DNA cloned into microcircles are demonstrated. A method of stepwise elongation or shortening of plasmids is discussed.

Keywords: Microcircles; Minicircles; Non-B-DNA structures; Non-canonical DNA.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

DNA Replication
DNA* / genetics
DNA, Circular* / genetics
Prospective Studies

Substances

DNA, Circular
DNA