Structure-switching aptamer triggering signal amplification strategy for tobramycin detection based on hybridization chain reaction and fluorescence synergism

Junyang Wang; Hongxia Li; Caiyi Du; Ying Li; Xinyue Ma; Chuanyu Yang; Wentao Xu; Chunyan Sun

doi:10.1016/j.talanta.2022.123318

Structure-switching aptamer triggering signal amplification strategy for tobramycin detection based on hybridization chain reaction and fluorescence synergism

Talanta. 2022 Jun 1:243:123318. doi: 10.1016/j.talanta.2022.123318. Epub 2022 Feb 15.

Authors

Junyang Wang¹, Hongxia Li¹, Caiyi Du¹, Ying Li¹, Xinyue Ma¹, Chuanyu Yang¹, Wentao Xu², Chunyan Sun³

Affiliations

¹ Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China.
² Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China. Electronic address: xuwentao@cau.edu.cn.
³ Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, China. Electronic address: sunchuny@jlu.edu.cn.

PMID: 35217273
DOI: 10.1016/j.talanta.2022.123318

Abstract

Based on hybridization chain reaction (HCR) and fluorescence synergism, a novel aptasensor for tobramycin was successfully constructed. Tobramycin competed with cDNA-FAM to bind aptamers immobilized on magnetic beads. After magnetic separation, the released cDNA-FAM acted as initiator to trigger HCR amplification, thus the fluorescence was significantly enhanced due to binding of SYBR Green Ⅰ (SGI) to the formed long double-stranded DNA and the synergistic fluorescence of FAM. In the absence of tobramycin, the initiator was magnetically separated and no HCR occurred, more importantly, graphene oxide can quench the fluorescence of excessive hairpins/SGI and cDNA-FAM, so almost no background signal was detected. This aptasensor can monitor tobramycin in the range of 0.3-50 μM with low detection limit of 17.37 nM. Due to the potential generality of structure-switching aptamers and effectiveness of fluorescence synergism, this enzyme-free amplification strategy can be extended to other applications by rational design of nucleic acid sequences.

Keywords: Fluorescence synergism; Hybridization chain reaction; Signal amplification; Structure-switching aptamer; Tobramycin.

MeSH terms

Aptamers, Nucleotide* / genetics
Biosensing Techniques*
DNA / genetics
Limit of Detection
Nucleic Acid Hybridization
Spectrometry, Fluorescence
Tobramycin

Substances

Aptamers, Nucleotide
DNA
Tobramycin