Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs

Zheying Min; Ke Zhong; Yuxin Luo; Yong Fan; Yang Yu

doi:10.3389/fcell.2022.840492

Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs

Front Cell Dev Biol. 2022 Feb 8:10:840492. doi: 10.3389/fcell.2022.840492. eCollection 2022.

Authors

Zheying Min^{1

2}, Ke Zhong¹, Yuxin Luo³, Yong Fan¹, Yang Yu^{2

3}

Affiliations

¹ Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
² Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
³ Stem Cell Research Center, Peking University Third Hospital, Beijing, China.

Abstract

Preimplantation embryo development is a precisely regulated process organized by maternally inherited and newly synthesized proteins. Recently, some studies have reported that blastocyst-like structures, named blastoids, can be generated from mouse ESCs (embryonic stem cells) or EPSCs (extended pluripotent stem cells). In this study, to explore the dynamic expression characteristics of proteins and their PTMs in mouse EPS blastoids, we revealed the protein expression profile of EPS blastoids and metabolite characteristics by TMT-based quantitative mass spectrometry (MS) strategy. Furthermore, the protein phosphorylation sites were identified to show the phosphoproteomic analysis in blastoids compared with mouse early embryos. Above all, our study revealed the protein expression profile of EPS blastoids compared with mouse embryos during preimplantation development and indicated that glucose metabolism is key to blastoid formation.

Keywords: blastoids; glucose metabolism; mEPSCs; phosphorylation; post translation modifications; proteome.