Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L

Ruolin Zhao; Hailiang Cheng; Qiaolian Wang; Limin Lv; Youping Zhang; Guoli Song; Dongyun Zuo

doi:10.3390/genes13020292

Identification of the CesA Subfamily and Functional Analysis of GhMCesA35 in Gossypium hirsutum L

Genes (Basel). 2022 Feb 1;13(2):292. doi: 10.3390/genes13020292.

Authors

Ruolin Zhao^{1

2}, Hailiang Cheng^{1

2}, Qiaolian Wang^{1

2}, Limin Lv^{1

2}, Youping Zhang^{1

2}, Guoli Song^{1

2}, Dongyun Zuo^{1

2}

Affiliations

¹ Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China.
² Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, China.

Abstract

The cellulose synthase genes control the biosynthesis of cellulose in plants. Nonetheless, the gene family members of CesA have not been identified in the newly assembled genome of Gossypiumhirsutum (AD1, HEBAU_NDM8). We identified 38 CesA genes in G. hirsutum (NDM8) and found that the protein sequence of GhMCesA35 is 100% identical to CelA1 in a previous study. It is already known that CelA1 is involved in cellulose biosynthesis in vitro. However, the function of this gene in vivo has not been validated. In this study, we verified the function of GhMCesA35 in vivo based on overexpressed Arabidopsis thaliana. In addition, we found that it interacted with GhCesA7 through the yeast two-hybrid assay. This study provides new insights for studying the biological functions of CesA genes in G. hirsutum, thereby improving cotton fiber quality and yield.

Keywords: CesA; cellulose; cotton; expression pattern; gene family.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis* / genetics
Cellulose
Cotton Fiber
Gossypium* / genetics
Phylogeny

Substances

Cellulose