Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices

Angel Baudon; Etienne Clauss-Creusot; Pascal Darbon; Ryan Patwell; Valery Grinevich; Alexandre Charlet

doi:10.1016/j.xpro.2022.101159

Calcium imaging and BAPTA loading of amygdala astrocytes in mouse brain slices

STAR Protoc. 2022 Feb 8;3(1):101159. doi: 10.1016/j.xpro.2022.101159. eCollection 2022 Mar 18.

Authors

Angel Baudon¹, Etienne Clauss-Creusot¹, Pascal Darbon¹, Ryan Patwell², Valery Grinevich², Alexandre Charlet¹

Affiliations

¹ Centre National de la Recherche Scientifique and University of Strasbourg, Institute of Cellular and Integrative Neuroscience, INCI CNRS UPR3212, 8, Allée du Général Rouvillois, Strasbourg, 67000 France.
² Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, University of Heidelberg, Mannheim, 68159 Germany.

Abstract

Astrocytes are glial cells that exhibit calcium signaling-mediated activity. Here, we present a protocol to monitor and manipulate astrocyte calcium activity from mouse amygdala slices. In the first part of this protocol, we describe the procedure of astrocyte calcium imaging. In the second part, we detail how to disrupt astrocyte calcium activity by patch-clamp-mediated loading of BAPTA. These two approaches are presented separately but they can also be used simultaneously to monitor the effects of disruption on an astrocyte network. For complete details on the use and execution of this protocol, please refer to Wahis et al. (2021).

Keywords: Cell Biology; Microscopy; Molecular/Chemical Probes; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amygdala / diagnostic imaging
Animals
Astrocytes* / metabolism
Calcium Signaling
Calcium* / metabolism
Calcium, Dietary
Egtazic Acid / analogs & derivatives
Mice

Substances

Calcium, Dietary
Egtazic Acid
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
Calcium