The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation

Kinga Kania; Anna Levytska; Anna Drożak; Borkowski Andrzej; Działak Paweł; Maksymilian Zienkiewicz

doi:10.1016/j.bbrep.2022.101220

The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation

Biochem Biophys Rep. 2022 Feb 7:30:101220. doi: 10.1016/j.bbrep.2022.101220. eCollection 2022 Jul.

Authors

Kinga Kania¹, Anna Levytska¹, Anna Drożak¹, Borkowski Andrzej², Działak Paweł², Maksymilian Zienkiewicz¹

Affiliations

¹ Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland.
² Faculty of Geology, Geophysics and Environmental Protection, AGH University of Science and Technology, Krakow, Poland.

Abstract

In this study, we have shown that transformation efficiency of Coccomyxa subellipsoidea C-169 obtained by electroporation can be significantly increased by either supra- or sub-optimal growth temperatures.

Keywords: Coccomyxa subellipsoidea C-169; Electrotransformation; Stable genome transformation.