Therapeutic potential of nitric oxide synthase inhibitor from natural sources for the treatment of ischemic stroke

Bader Alshehri; Rajendran Vijayakumar; Subramanian Senthilkumar; Ahmed Ismail; Ahmed Abdel-Hadi; Ranjay K Choudhary; Kamal S Albenasy; Saeed Banawas; Mohammed A Alaidarous; Palanisamy Manikandan

doi:10.1016/j.sjbs.2021.10.003

Therapeutic potential of nitric oxide synthase inhibitor from natural sources for the treatment of ischemic stroke

Saudi J Biol Sci. 2022 Feb;29(2):984-991. doi: 10.1016/j.sjbs.2021.10.003. Epub 2021 Oct 9.

Authors

Affiliations

¹ Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia, Kingdom of Saudi Arabia.
² Department of Biology, College of Science, Majmaah University, Majmaah 11952, Saudi Arabia, Kingdom of Saudi Arabia.
³ Department of Medicine, College of Medicine and Health Science, Jigjiga University, Ethiopia.
⁴ Department of Public Health, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia, Kingdom of Saudi Arabia.
⁵ Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
⁶ Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt.
⁷ Department of Biomedical Sciences, Oregon State University, Corvallis, OR 97331, USA.
⁸ Greenlink Analytical and Research Laboratory India Private Limited, Coimbatore 641 014, India.

Abstract

Nitric oxide (NO) is one of the major signalling molecules in the mammalian body playing critical role in regulation of blood pressure, cardiovascular disease including stroke, immune activation, neuronal and cell communication. Moreover, hyper production of NO by the activity of nitric oxide synthase (NOS) involved in neuropathic pain, neurodegenerative disorders and stroke. Hence, the search on small molecules from the natural sources for the inhibition of NOS is desirable in therapeutic point of view. The elevated level of NO caused by NOS enzyme become a novel target in finding new inhibitors from natural sources as antistroke agents. The present study focuses on the molecular docking of quercetin and its analogues against NOS. The active site of the enzyme was docked with the ligand and pharmacological properties were analysed. From this result, we suggest the therapeutic property of quercetin and its analogues against NOS.

Keywords: Ischemic stroke; Molecular docking; Natural sources; Nitric oxide synthase inhibitor.